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KMID : 0613319960020010018
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Journal of the Korean Bone and Joint Tumor Soceity
1996 Volume.2 No. 1 p.18 ~ p.26
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Comparson of Osteogenic Potentials of Deep Freezed, Boiled and Autoclaved Autogenous Long Bone Graft in Rabbits
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Chang Han
Kim Yong-Sik
Lee Nam-Ki
Kim Seung-Ki
Lee Gi-Haeng
Koh Hae-Suk
Cho Hyung-Rae
Rhee Seung-Koo
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Abstract
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We investigated the effect of deep-freezed, boiled and autoclaved autogenous long bone graft on the incorporation of the radial diaphyseal reconstruction in 30 rabbits by radiogram and histology for 8 weeks. Immediate histologic changes of 1cm of resected long bone treated by deep freezing, boiling and autoclaving in each 2 rabbits were also observed as control. Resected, boiled (95℃ for 5 min.) and reimplanted bone was compared with resected, autoclaved (131℃ for 5 min.) and reimplanted bone, and resected, deep freezed with litrogen for 5 minutes and thawing in saline and reimplanted bone in the reconstruction of bilateral radial defcots in each of 8, and in total 24 adult rabbits. The results were as follows:
1. Immediate histologic changes showed that intracortical osteocytes in lacunae were patially necrotized and the cortex were faintly stained with they Masson trichrome stain in both boiled and deep freezed groups, while they completely necrotized and their cortex stained more weakly with Masson trichrome stain in autoclaved group which means less amount of collagen and protein in cortex of long bone. 2.Radiographies at 8 weeks showed complete union with marked incorporation and external callus formation in all boiled and freezed groups,whereas there was delayed union in four of sixteen (25%)in autoclaved group.Histologically,at 8 weeks after boiled and freezed,more intense incorpo-ration with new bone formation and neovascularization were observed,whereas transverse clefts con-sisted with delayed union in 4 cases of autoclaved group(25%) were observed at osteotomy site. Through these studies, the boilod and deeply freezed bones acted as an osteoinductive material as well as osteoconductive,but the autoclaved bone only as osteoconductive.Though boilod and deeply freezed bone showed higher osteogenic potentials than autoclaved bone,the necrotizing effect on cortical and boiled bone was inferior to that of autoclaved.Thus the deeply freezed bone can be used for the treatment of appressive benign or less malignant bone tumor not involving cortical bone, but the autoclaved bone supplemented with bone graft for the treatment of malignant bone tumor involv-ing cortex of long bone.
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KEYWORD
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Osteogenesis, Deep freezing with liquid N2, Boiled bone, Autoclaved bone, Limb sal-Vage operation
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